Inhibitory effect of Turkish Rosmarinus officinalis L. on acetylcholinesterase and butyrylcholinesterase enzymes

Ilkay Orhan; Sinem Aslan; Murat Kartal; Bilge Şener; K Hüsnü Can Başer

doi:10.1016/j.foodchem.2007.11.023

Inhibitory effect of Turkish Rosmarinus officinalis L. on acetylcholinesterase and butyrylcholinesterase enzymes

Food Chem. 2008 May 15;108(2):663-8. doi: 10.1016/j.foodchem.2007.11.023. Epub 2007 Nov 19.

Authors

Ilkay Orhan¹, Sinem Aslan², Murat Kartal², Bilge Şener³, K Hüsnü Can Başer⁴

Affiliations

¹ Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey. Electronic address: iorhan@gazi.edu.tr.
² Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey.
³ Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, Turkey.
⁴ Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskişehir, Turkey.

PMID: 26059146
DOI: 10.1016/j.foodchem.2007.11.023

Abstract

In the current study, we have tested acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the petroleum ether, ethyl acetate, chloroform, and methanol extracts, rosmarinic acid as well as the essential oil obtained from Rosmarinus officinalis L. growing in Turkey by a spectrophotometric method of Ellman using ELISA microplate-reader at 0.2,0.5, and 1.0mg/mL concentrations. In addition, quantification of rosmarinic acid, a common phenolic acid found in rosemary, was carried out by reversed-phase HPLC in the methanolic extract of the plant, which was found to have 12.21±0.95% (122.1±9.5mg/g extract) of rosmarinic acid. Rosmarinic acid was also tested for its AChE and BChE inhibitory effect and found to cause 85.8% of inhibition against AChE at only 1.0mg/mL. Besides, the essential oil was analyzed by GC-MS technique, which was shown to be dominated by 1,8-cineol (44.42%) and followed by α-pinene (12.57%).

Keywords: Acetylcholinesterase; Butyrylcholinesterase; Essential oil; Rosmarinic acid; Rosmarinus officinalis L..