In Situ Detection of Integrin Ligands

Curr Protoc Cell Biol. 2014 Dec 11;65:9.7.1-17. doi: 10.1002/0471143030.cb0907s65.

Abstract

Integrins are cell surface receptors for cell adhesion. Integrin-mediated cell adhesion regulates various cellular processes, including cell survival, migration, proliferation, and differentiation. In vivo, ligands for integrins are immobilized within extracellular matrices, insoluble sheet-like or fibrous supramolecular complexes that associate with or surround cells. To better understand the molecular basis of integrin-mediated regulation of cellular behavior in vivo, it is of critical importance to collect information regarding the activities as well as spatial distributions of integrin ligands in situ. This unit describes a protocol for detecting the spatial distribution of the complement of integrin ligands in situ by overlaying soluble recombinant integrins.

Keywords: basement membrane; cell adhesion; extracellular matrix (ECM); integrin.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Adhesion
  • Extracellular Matrix / chemistry*
  • Extracellular Matrix / metabolism
  • Humans
  • Integrins / chemistry*
  • Integrins / metabolism
  • Ligands

Substances

  • Integrins
  • Ligands