Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay

Sci Rep. 2015 Jun 10;5:11333. doi: 10.1038/srep11333.

Abstract

G-protein-coupled receptors (GPCRs) are one of the most important drug targets, and anti-GPCR monoclonal antibody (mAb) is an essential tool for functional analysis of GPCRs. However, it is very difficult to develop GPCR-specific mAbs due to difficulties in production of recombinant GPCR antigens, and lack of efficient mAb screening method. Here we describe a novel approach for the production of mAbs against GPCR using two original methods, bilayer-dialysis method and biotinylated liposome-based interaction assay (BiLIA), both of which are developed using wheat cell-free protein synthesis system and liposome technology. Using bilayer-dialysis method, various GPCRs were successfully synthesized with quality and quantity sufficient for immunization. For selection of specific mAb, we designed BiLIA that detects interaction between antibody and membrane protein on liposome. BiLIA prevented denaturation of GPCR, and then preferably selected conformation-sensitive antibodies. Using this approach, we successfully obtained mAbs against DRD1, GHSR, PTGER1 and T1R1. With respect to DRD1 mAb, 36 mouse mAbs and 6 rabbit mAbs were obtained which specifically recognized native DRD1 with high affinity. Among them, half of the mAbs were conformation-sensitive mAb, and two mAbs recognized extracellular loop 2 of DRD1. These results indicated that this approach is useful for GPCR mAb production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Antibody Affinity / immunology
  • Biotinylation
  • Cell-Free System
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Epitope Mapping
  • Epitopes / immunology
  • Humans
  • Liposomes / immunology
  • Mice
  • Rabbits
  • Receptors, Dopamine D1 / immunology*
  • Receptors, G-Protein-Coupled / immunology*
  • Receptors, Ghrelin / immunology*
  • Receptors, Prostaglandin E, EP1 Subtype / immunology*
  • Recombinant Proteins / chemical synthesis
  • Recombinant Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • DRD1 protein, human
  • Epitopes
  • Liposomes
  • PTGER1 protein, human
  • Receptors, Dopamine D1
  • Receptors, G-Protein-Coupled
  • Receptors, Ghrelin
  • Receptors, Prostaglandin E, EP1 Subtype
  • Recombinant Proteins
  • taste receptors, type 1