Allergen Microarray Indicates Pooideae Sensitization in Brazilian Grass Pollen Allergic Patients

PLoS One. 2015 Jun 11;10(6):e0128402. doi: 10.1371/journal.pone.0128402. eCollection 2015.


Background: Grass pollen, in particular from Lolium multiflorum is a major allergen source in temperate climate zones of Southern Brazil. The IgE sensitization profile of Brazilian grass pollen allergic patients to individual allergen molecules has not been analyzed yet.

Objective: To analyze the IgE sensitization profile of a Brazilian grass pollen allergic population using individual allergen molecules.

Methods: We analyzed sera from 78 grass pollen allergic patients for the presence of IgE antibodies specific for 103 purified micro-arrayed natural and recombinant allergens by chip technology. IgE-ELISA inhibition experiments with Lolium multiflorum, Phleum pratense extracts and a recombinant fusion protein consisting of Phl p 1, Phl p 2, Phl p 5 and Phl p 6 were performed to investigate cross-reactivities.

Results: Within the Brazilian grass pollen allergic patients, the most frequently recognized allergens were Phl p 1 (95%), Phl p 5 (82%), Phl p 2 (76%) followed by Phl p 4 (64%), Phl p 6 (45%), Phl p 11 (18%) and Phl p 12 (18%). Most patients were sensitized only to grass pollen allergens but not to allergens from other sources. A high degree of IgE cross-reactivity between Phleum pratense, Lolium multiflorum and the recombinant timothy grass fusion protein was found.

Conclusions: Component-resolved analysis of sera from Brazilian grass pollen allergic patients reveals an IgE recognition profile compatible with a typical Pooideae sensitization. The high degree of cross-reactivity between Phleum pratense and Lolium multiflorum allergens suggests that diagnosis and immunotherapy can be achieved with timothy grass pollen allergens in the studied population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Allergens / immunology*
  • Brazil
  • Cross Reactions / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Hypersensitivity / immunology
  • Hypersensitivity / pathology*
  • Immunoglobulin E / immunology
  • Immunoglobulin E / metabolism
  • Lolium / metabolism*
  • Male
  • Phleum / metabolism
  • Plant Proteins / genetics
  • Plant Proteins / immunology
  • Plant Proteins / metabolism
  • Pollen / immunology*
  • Protein Array Analysis
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Skin Tests


  • Allergens
  • Plant Proteins
  • Recombinant Fusion Proteins
  • Immunoglobulin E