doi: 10.1002/9780470942390.mo140061.
High-Resolution Respirometry for Mitochondrial Characterization of Ex Vivo Mouse Tissues
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- PMID: 26069081
- DOI: 10.1002/9780470942390.mo140061
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High-Resolution Respirometry for Mitochondrial Characterization of Ex Vivo Mouse Tissues
Curr Protoc Mouse Biol.
.
Abstract
This article describes methodologies to examine mitochondrial respiration in fresh preparations of mouse tissues, including skeletal muscle, heart, liver, white and brown adipose tissue, and brain. Reference values and tips to maximize experimental efficiencies are also provided. Finally, correction methods and complementary techniques to properly interpret the results are presented and contrasted.
Keywords: bioenergetics; mitochondrial physiology; respiratory complexes.
Copyright © 2015 John Wiley & Sons, Inc.
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