Defining the phenotype of neutrophils following reverse migration in zebrafish

J Leukoc Biol. 2015 Dec;98(6):975-81. doi: 10.1189/jlb.3MA0315-105R. Epub 2015 Jun 12.


Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.

Keywords: chemotaxis; granulocytes; infection; inflammation; phagocytosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified / genetics
  • Animals, Genetically Modified / immunology
  • Cell Movement / genetics
  • Cell Movement / immunology*
  • Inflammation / genetics
  • Inflammation / immunology
  • Neutrophils / immunology*
  • Staphylococcus aureus / immunology
  • Zebrafish / genetics
  • Zebrafish / immunology*