Development of a human live attenuated West Nile infectious DNA vaccine: conceptual design of the vaccine candidate

Virology. 2015 Oct;484:59-68. doi: 10.1016/j.virol.2015.04.027. Epub 2015 Jun 10.

Abstract

West Nile virus has become an important epidemiological problem attracting significant attention of health authorities, mass media, and the public. Although there are promising advancements toward addressing the vaccine need, the perspectives of the commercial availability of the vaccine remain uncertain. To a large extent this is due to lack of a sustained interest for further commercial development of the vaccines already undergoing the preclinical and clinical development, and a predicted insignificant cost effectiveness of mass vaccination. There is a need for a safe, efficacious and cost effective vaccine, which can improve the feasibility of a targeted vaccination program. In the present report, we summarize the background, the rationale, and the choice of the development pathway that we selected for the design of a live attenuated human West Nile vaccine in a novel infectious DNA format.

Keywords: Human; Infectious DNA; Mice; Pathogenicity; Vaccine; Virulence; West Nile virus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Line
  • Female
  • Mice
  • Vaccines, Attenuated / administration & dosage
  • Vaccines, Attenuated / adverse effects
  • Vaccines, Attenuated / genetics
  • Vaccines, Attenuated / isolation & purification
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / adverse effects*
  • Vaccines, DNA / genetics
  • Vaccines, DNA / isolation & purification
  • Viral Load
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / adverse effects*
  • Viral Vaccines / genetics
  • Viral Vaccines / isolation & purification
  • Virulence
  • West Nile virus / genetics
  • West Nile virus / growth & development*

Substances

  • Vaccines, Attenuated
  • Vaccines, DNA
  • Viral Vaccines