TSG (2,3,5,4'-Tetrahydroxystilbene-2-O- β -D-glucoside) from the Chinese Herb Polygonum multiflorum Increases Life Span and Stress Resistance of Caenorhabditis elegans

Oxid Med Cell Longev. 2015:2015:124357. doi: 10.1155/2015/124357. Epub 2015 May 5.


2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) was isolated from Polygonum multiflorum, a plant which is traditionally used as an anti-ageing drug. We have analysed ageing-related effects of TSG in the model organism C. elegans in comparison to resveratrol. TSG exerted a high antioxidative capacity both in a cell-free assay and in the nematode. The antioxidative capacity was even higher compared to resveratrol. Presumably due to its antioxidative effects, treatment with TSG decreased the juglone-mediated induction of the antioxidative enzyme SOD-3; the induction of the GST-4 by juglone was diminished slightly. TSG increased the resistance of C. elegans against lethal thermal stress more prominently than resveratrol (50 μM TSG increased mean survival by 22.2%). The level of the ageing pigment lipofuscin was decreased after incubation with the compound. TSG prolongs the mean, median, and maximum adult life span of C. elegans by 23.5%, 29.4%, and 7.2%, respectively, comparable to the effects of resveratrol. TSG-mediated extension of life span was not abolished in a DAF-16 loss-of-function mutant strain showing that this ageing-related transcription factor is not involved in the effects of TSG. Our data show that TSG possesses a potent antioxidative capacity, enhances the stress resistance, and increases the life span of the nematode C. elegans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Caenorhabditis elegans / drug effects
  • Caenorhabditis elegans / enzymology
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / metabolism
  • Drugs, Chinese Herbal / chemistry
  • Drugs, Chinese Herbal / pharmacology*
  • Fallopia multiflora / chemistry*
  • Free Radical Scavengers / pharmacology
  • Glucosides / chemistry
  • Glucosides / pharmacology*
  • Glutathione Transferase / metabolism
  • Heat-Shock Response / drug effects
  • Longevity / drug effects*
  • Mutation / genetics
  • Reactive Oxygen Species / metabolism
  • Stilbenes / chemistry
  • Stilbenes / pharmacology*
  • Stress, Physiological / drug effects*
  • Superoxide Dismutase / metabolism


  • Antioxidants
  • Caenorhabditis elegans Proteins
  • Drugs, Chinese Herbal
  • Free Radical Scavengers
  • Glucosides
  • Reactive Oxygen Species
  • Stilbenes
  • 2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside
  • Superoxide Dismutase
  • Glutathione Transferase