Derivation of Cinnamon Blocks Leukocyte Attachment by Interacting with Sialosides

Wei-Ling Lin; Shih-Yun Guu; Chan-Chuan Tsai; Ekambaranellore Prakash; Mohan Viswaraman; Hsing-Bao Chen; Chuan-Fa Chang

doi:10.1371/journal.pone.0130389

Derivation of Cinnamon Blocks Leukocyte Attachment by Interacting with Sialosides

PLoS One. 2015 Jun 15;10(6):e0130389. doi: 10.1371/journal.pone.0130389. eCollection 2015.

Authors

Wei-Ling Lin¹, Shih-Yun Guu², Chan-Chuan Tsai³, Ekambaranellore Prakash⁴, Mohan Viswaraman⁴, Hsing-Bao Chen⁵, Chuan-Fa Chang⁶

Affiliations

¹ Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
² Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan.
³ Department of Pathology, Pingtung Christian Hospital, Pingtung 900, Taiwan.
⁴ Indus Biotech Private Limited, Kondhwa, Pune 411048, India.
⁵ Division of Colorectal Surgery, Department of Surgery, E-DA Hospital, Kaohsiung 82445, Taiwan.
⁶ Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Center of Infectious Disease and Signaling Research, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan.

Abstract

Molecules derived from cinnamon have demonstrated diverse pharmacological activities against infectious pathogens, diabetes and inflammatory diseases. This study aims to evaluate the effect of the cinnamon-derived molecule IND02 on the adhesion of leukocytes to host cells. The anti-inflammatory ability of IND02, a pentameric procyanidin type A polyphenol polymer isolated from cinnamon alcohol extract, was examined. Pretreatment with IND02 significantly reduced the attachment of THP-1 cells or neutrophils to TNF-α-activated HUVECs or E-selectin/ICAM-1, respectively. IND02 also reduced the binding of E-, L- and P-selectins with sialosides. Furthermore, IND02 could agglutinate human red blood cells (RBC), and the agglutination could be disrupted by sialylated glycoprotein. Our findings demonstrate that IND02, a cinnamon-derived compound, can interact with sialosides and block the binding of selectins and leukocytes with sialic acids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agglutination / drug effects
Anti-Inflammatory Agents / pharmacology
Cell Adhesion / drug effects*
Cell Line, Tumor
Cinnamomum zeylanicum / metabolism*
Drugs, Chinese Herbal / pharmacology
E-Selectin / metabolism
Endothelium, Vascular / metabolism
Erythrocyte Aggregation / drug effects*
Erythrocytes / drug effects
Human Umbilical Vein Endothelial Cells / metabolism*
Humans
Inflammation / drug therapy
Intercellular Adhesion Molecule-1 / metabolism
N-Acetylneuraminic Acid / metabolism
Neutrophils / metabolism*
Orthomyxoviridae / metabolism
Proanthocyanidins / pharmacology*
Protein Binding / drug effects
Tumor Necrosis Factor-alpha / metabolism

Substances

Anti-Inflammatory Agents
Drugs, Chinese Herbal
E-Selectin
IND02 polymer
Proanthocyanidins
Tumor Necrosis Factor-alpha
Intercellular Adhesion Molecule-1
N-Acetylneuraminic Acid

Grants and funding

This work was supported by the Center of Infectious Disease and Signaling Research of NCKU and the Ministry of Science and Technology of Taiwan (NSC 102-2321-B-006-006, http://www.most.gov.tw/). Indus Biotech Private Limited provided support in the form of salaries for authors Ekambaranellore Prakash and Mohan Viswaraman, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ‘author contributions’ section. This does not alter the authors' adherence to PLOS ONE policies on sharing data and materials.