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. 2015 Jun 16:5:11355.
doi: 10.1038/srep11355.

Cytokine profile in severe Gram-positive and Gram-negative abdominal sepsis

Maja Surbatovic  1 , Nada Popovic  2 , Danilo Vojvodic  3 , Ivan Milosevic  2 , Gordana Acimovic  4 , Milan Stojicic  2 , Milic Veljovic  1 , Jasna Jevdjic  5 , Dragan Djordjevic  1 , Sonja Radakovic  6
Affiliations

Cytokine profile in severe Gram-positive and Gram-negative abdominal sepsis

Maja Surbatovic et al. Sci Rep. .

Abstract

Sepsis is a principal cause of death in critical care units worldwide and consumes considerable healthcare resources. The aim of our study was to determine whether the early cytokine profile can discriminate between Gram-positive and Gram-negative bacteraemia (GPB and GNB, respectively) and to assess the prognostic value regarding outcome in critically ill patients with severe abdominal sepsis. The outcome measure was hospital mortality. Blood samples were obtained from 165 adult patients with confirmed severe abdominal sepsis. Levels of the proinflammatory mediators TNF-α, IL-8, IL-12 and IFN-γ and the anti-inflammatory mediators IL-1ra, IL-4, IL-10 and TGF-β1 were determined and correlated with the nature of the bacteria isolated from the blood culture and outcome. The cytokine profile in our study indicated that the TNF-α levels were 2-fold, IL-8 were 3.3-fold, IFN-γ were 13-fold, IL-1ra were 1.05-fold, IL-4 were 1.4-fold and IL-10 were 1.83-fold higher in the GNB group compared with the GPB group. The TNF-α levels were 4.7-fold, IL-8 were 4.6-fold, IL-1ra were 1.5-fold and IL-10 were 3.3-fold higher in the non-survivors compared with the survivors.

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Figures

Figure 1
Figure 1. Serum proinflammatory cytokine TNF-α, IL-8, IL-12 and IFN-γ levels according to the nature of the bacteraemia.
Figure 2
Figure 2. Serum anti-inflammatory cytokine IL-1ra, IL-4, IL-10 and TGF-β1 levels according to the nature of the bacteraemia.
Figure 3
Figure 3. Area under the ROC curve for IL-1ra, IL-8 and IL-10 individual cytokine measurements and the presence of Gram-positive abdominal sepsis (3A); TNF-α, IL-8, IL-12 (3B) and IL-1ra, IL-10 (3C) measurements and Gram-negative abdominal sepsis.
Figure 4
Figure 4. Serum proinflammatory cytokine TNF-α, IL-8, IL-12 and IFN-γ levels according to the outcome.
Figure 5
Figure 5. Serum anti-inflammatory cytokine IL-1ra, IL-4, IL-10 and TGF-β1 levels according to the outcome.
Figure 6
Figure 6. Area under the ROC curve for TNF-α, IL-8 (6A) and IL-1ra, IL-4, IL-10 (6B) measurements and the outcome.
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