Breast regression protein-39 is not required for experimental autoimmune encephalomyelitis induction

Clin Immunol. 2015 Oct;160(2):133-41. doi: 10.1016/j.clim.2015.06.004. Epub 2015 Jun 12.


Increasing evidence points to a role for chitinase 3-like 1 (CHI3L1) in multiple sclerosis (MS). Here, we aimed to explore the potential involvement of CHI3L1 in the animal model of MS, experimental autoimmune encephalomyelitis (EAE). EAE was induced by immunization with MOG 35-55 peptide in wild-type (WT) and knock-out (KO) mice for breast regression protein 39 (BRP-39), the mouse homologue of human CHI3L1. Immunological responses in splenocytes were assessed by means of polyclonal and antigen-specific proliferation assays. Central nervous system pathology and chitinase gene expression were also investigated. BRP-39 expression was increased in WT MOG 35-55-immunized mice compared to saline-immunized controls. No differences were found between WT and BRP-39 KO mice regarding EAE clinical course, day of disease onset, mortality rate, splenocyte proliferative responses or histopathological findings. These results do not support a role of BRP-39 in the pathogenesis of EAE.

Keywords: BRP-39; EAE; Multiple sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / metabolism
  • Chitinase-3-Like Protein 1
  • Chitinases / genetics
  • Disease Progression
  • Encephalomyelitis, Autoimmune, Experimental / genetics*
  • Encephalomyelitis, Autoimmune, Experimental / immunology
  • Gene Expression Profiling
  • Glycoproteins / genetics*
  • Hexosaminidases / genetics
  • Immunization
  • Lectins / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microglia / metabolism
  • Myelin-Oligodendrocyte Glycoprotein / immunology
  • Peptide Fragments / immunology
  • RNA, Messenger / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spinal Cord / metabolism*
  • Spinal Cord / pathology
  • beta-N-Acetylhexosaminidases / genetics


  • Chil1 protein, mouse
  • Chitinase-3-Like Protein 1
  • Glycoproteins
  • Lectins
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • RNA, Messenger
  • myelin oligodendrocyte glycoprotein (35-55)
  • Hexosaminidases
  • chitotriosidase
  • AMCase, mouse
  • Chitinases
  • Chil3 protein, mouse
  • beta-N-Acetylhexosaminidases