Retinal Flip in Rhodopsin Activation?

Biophys J. 2015 Jun 16;108(12):2767-70. doi: 10.1016/j.bpj.2015.04.040.

Abstract

Rhodopsin is a well-characterized structural model of a G protein-coupled receptor. Photoisomerization of the covalently bound retinal triggers activation. Surprisingly, the x-ray crystal structure of the active Meta-II state has a 180° rotation about the long-axis of the retinal polyene chain. Unbiased microsecond-timescale all-atom molecular dynamics simulations show that the retinal cofactor can flip back to the orientation observed in the inactive state of rhodopsin under conditions favoring the Meta-I state. Our results provide, to our knowledge, the first evidence from molecular dynamics simulations showing how rotation of the retinal ligand within its binding pocket can occur in the activation mechanism of rhodopsin.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Lipid Bilayers / chemistry
  • Molecular Dynamics Simulation*
  • Molecular Sequence Data
  • Rhodopsin / chemistry*
  • Rotation

Substances

  • Lipid Bilayers
  • Rhodopsin