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. 2015 Jun 18:4:e06938.
doi: 10.7554/eLife.06938.

Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization

Francesca Incardona  1 , M Mehdi Doroudchi  1 , Nawfal Ismail  1 , Alberto Carreno  1 , Erin Griner  2 , Minyoung Anna Lim  3 ; Reproducibility Project: Cancer Biology
Collaborators, Affiliations

Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization

Francesca Incardona et al. Elife. .

Abstract

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from 'Interactions between cancer stem cells and their niche govern metastatic colonization' by Malanchi and colleagues, published in Nature in 2012 (Malanchi et al., 2012). The key experiments that will be replicated are those reported in Figures 2H, 3A, 3B, and S13. In these experiments, Malanchi and colleagues analyze messenger RNA levels of periostin (POSTN) in pulmonary fibroblasts, endothelial cells, and immune cells isolated from mice with micrometastases to determine which cell type is producing POSTN in the metastatic niche (Figure 2H; Malanchi et al., 2012). Additionally, they examine MMTV-PyMT control or POSTN null mice to test the effect of POSTN on primary tumor growth and metastasis (Figures 3A, 3B, and S13; Malanchi et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange, and the results of the replications will be published in eLife.

Keywords: Postn; Reproducibility Project: Cancer Biology; breast cancer; developmental biology; metastasis; methodology; mouse; stem cells.

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Conflict of interest statement

FI: This is a Science Exchange associated lab.

MMD: This is a Science Exchange associated lab.

NI: This is a Science Exchange associated lab.

AC: This is a Science Exchange associated lab.

RP:CB: EI, FT, JL, and NP are employed by and hold shares in Science Exchange Inc.

The other authors declare that no competing interests exist.

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The Reproducibility Project: Cancer Biology is funded by the Laura and John Arnold Foundation, provided to the Center for Open Science in collaboration with Science Exchange. The funder had no role in study design or the decision to submit the work for publication.