LOV-based reporters for fluorescence imaging

Curr Opin Chem Biol. 2015 Aug;27:39-45. doi: 10.1016/j.cbpa.2015.05.011. Epub 2015 Jun 15.


Chromophore-binding domains from plant and bacterial photoreceptor proteins have recently gathered increasing attention as new sources of genetically encoded fluorescent proteins (FPs). In particular, FPs based on the flavin-binding LOV (light, oxygen, or voltage sensing) domain offer advantages over green fluorescent protein (GFP) owing to their smaller size, pH and thermal stability, utility under anaerobic conditions and their ability to generate reactive oxygen species. This review focuses on the potential applications of this emerging class of fluorescent reporters, discusses the advantages and limitations of LOV-based FPs, whilst offering insights regarding the further development of this technology for bioimaging and photodynamic therapy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Biosensing Techniques / methods*
  • Dinitrocresols / chemistry*
  • Fluorescent Dyes / chemistry*
  • Green Fluorescent Proteins / chemistry*
  • Green Fluorescent Proteins / genetics
  • Models, Molecular
  • Molecular Imaging / methods*
  • Oxygen / chemistry
  • Protein Binding
  • Protein Stability
  • Ultraviolet Rays


  • Bacterial Proteins
  • Dinitrocresols
  • Fluorescent Dyes
  • green fluorescent protein, Aequorea victoria
  • Green Fluorescent Proteins
  • 4,6-dinitro-o-cresol
  • Oxygen