Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules

Adv Healthc Mater. 2015 Aug 26;4(12):1802-12. doi: 10.1002/adhm.201500334. Epub 2015 Jun 18.


Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

Keywords: drug delivery; hydrogels; pharmaceutical excipients; protein stability; trehalose.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry*
  • Delayed-Action Preparations*
  • Drug Stability
  • Freeze Drying
  • Hydrogels / chemistry*
  • Hydrogen Bonding
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Trehalose / chemistry*


  • Biocompatible Materials
  • Delayed-Action Preparations
  • Hydrogels
  • Trehalose