Overexpression of miR-206 suppresses glycolysis, proliferation and migration in breast cancer cells via PFKFB3 targeting

Biochem Biophys Res Commun. 2015 Aug 7;463(4):1115-21. doi: 10.1016/j.bbrc.2015.06.068. Epub 2015 Jun 17.


miRNAs, sorting as non-coding RNAs, are differentially expressed in breast tumor and act as tumor promoters or suppressors. miR-206 could suppress the progression of breast cancer, the mechanism of which remains unclear. The study here was aimed to investigate the effect of miR-206 on human breast cancers. We found that miR-206 was down-regulated while one of its predicted targets, 6-Phosphofructo-2-kinase (PFKFB3) was up-regulated in human breast carcinomas. 17β-estradiol dose-dependently decreased miR-206 expression as well as enhanced PFKFB3 mRNA and protein expression in estrogen receptor α (ERα) positive breast cancer cells. Furthermore, we identified that miR-206 directly interacted with 3'-untranslated region (UTR) of PFKFB3 mRNA. miR-206 modulated PFKFB3 expression in MCF-7, T47D and SUM159 cells, which was influenced by 17β-estradiol depending on ERα expression. In addition, miR-206 overexpression impeded fructose-2,6-bisphosphate (F2,6BP) production, diminished lactate generation and reduced cell proliferation and migration in breast cancer cells. In conclusion, our study demonstrated that miR-206 regulated PFKFB3 expression in breast cancer cells, thereby stunting glycolysis, cell proliferation and migration.

Keywords: 6-Phosphofructo-2-kinase; Breast cancer; Glycolysis; Migration; Proliferation; miR-206.

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation*
  • Glycolysis*
  • HEK293 Cells
  • Humans
  • MicroRNAs / genetics*
  • Neoplasm Metastasis*
  • Phosphofructokinase-2 / metabolism*
  • Triple Negative Breast Neoplasms / metabolism
  • Triple Negative Breast Neoplasms / pathology*


  • MIRN206 microRNA, human
  • MicroRNAs
  • PFKFB3 protein, human
  • Phosphofructokinase-2