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. 2015;16 Suppl 7(Suppl 7):S4.
doi: 10.1186/1471-2164-16-S7-S4. Epub 2015 Jun 11.

A Genome-Wide Systems Analysis Reveals Strong Link Between Colorectal Cancer and Trimethylamine N-oxide (TMAO), a Gut Microbial Metabolite of Dietary Meat and Fat

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A Genome-Wide Systems Analysis Reveals Strong Link Between Colorectal Cancer and Trimethylamine N-oxide (TMAO), a Gut Microbial Metabolite of Dietary Meat and Fat

Rong Xu et al. BMC Genomics. .
Free PMC article


Background: Dietary intakes of red meat and fat are established risk factors for both colorectal cancer (CRC) and cardiovascular disease (CVDs). Recent studies have shown a mechanistic link between TMAO, an intestinal microbial metabolite of red meat and fat, and risk of CVDs. Data linking TMAO directly to CRC is, however, lacking. Here, we present an unbiased data-driven network-based systems approach to uncover a potential genetic relationship between TMAO and CRC.

Materials and methods: We constructed two different epigenetic interaction networks (EINs) using chemical-gene, disease-gene and protein-protein interaction data from multiple large-scale data resources. We developed a network-based ranking algorithm to ascertain TMAO-related diseases from EINs. We systematically analyzed disease categories among TMAO-related diseases at different ranking cutoffs. We then determined which genetic pathways were associated with both TMAO and CRC.

Results: We show that CVDs and their major risk factors were ranked highly among TMAO-related diseases, confirming the newly discovered mechanistic link between CVDs and TMAO, and thus validating our algorithms. CRC was ranked highly among TMAO-related disease retrieved from both EINs (top 0.02%, #1 out of 4,372 diseases retrieved based on Mendelian genetics and top 10.9% among 882 diseases based on genome-wide association genetics), providing strong supporting evidence for our hypothesis that TMAO is genetically related to CRC. We have also identified putative genetic pathways that may link TMAO to CRC, which warrants further investigation. Through systematic disease enrichment analysis, we also demonstrated that TMAO is related to metabolic syndromes and cancers in general.

Conclusions: Our genome-wide analysis demonstrates that systems approaches to studying the epigenetic interactions among diet, microbiome metabolisms, and disease genetics hold promise for understanding disease pathogenesis. Our results show that TMAO is genetically associated with CRC. This study suggests that TMAO may be an important intermediate marker linking dietary meat and fat and gut microbiota metabolism to risk of CRC, underscoring opportunities for the development of new gut microbiome-dependent diagnostic tests and therapeutics for CRC.


Figure 1
Figure 1
Finding TMAO-related diseases. Transforming GDNs into epigenetic interaction network (EINs) and finding TMAO-related diseases from transformed EINs.
Figure 2
Figure 2
Finding interplaying genetic ways for TMAO and CRC. Finding putative genetic pathways linking TMAO to CRC.
Figure 3
Figure 3
Disease enrichment analysis for TMAO-related diseases at 10 different ranking cutoffs. Enrichment of disease classes among TMAO-related diseases at ten ranking cutoffs. TMAO-related diseases were retrieved from the OMIM-based network. The other nine disease classes (not shown) were not enriched among top-ranked diseases.

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