The interactome of soybean GmWRKY53 using yeast 2-hybrid library screening to saturation

Plant Signal Behav. 2015;10(7):e1028705. doi: 10.1080/15592324.2015.1028705.


Soybean GmWRKY53 functions in both biotic and abiotic stress signaling. Using GmWRKY53 as a bait yeast 2-hybrid library screening to saturation isolated multiple independent fragments for many interacting proteins, enabling delineation of minimal interacting domains and computation of a confidence score. Multiple independent clones coding for the LATE ELONGATED HYPOCOTYL clock protein GmLCL2 (MYB114) were isolated and the binding site for GmWRKY53 was mapped to 90 amino acids separate from the MYB domain. This suggests a direct input from the clock on GmWRKY53 activity. The GmWRKY53-interacting proteins also included 3 water stress-inducible AP2/ERF transcription factors. One of these (Glyma03g26310) is one of the most strongly water stress induced genes in soybean roots, suggesting that GmWRKY53/ERF complexes regulate water stress responses.

Keywords: ERF transcription factor; GTPase accelerating proteins, GAP; LATE ELONGATED HYPOCOTYL, LHY; WRKY transcription factor; bright yellow 2, BY-2; clock protein; drought; guanine nucleotide exchange factor, GEF; protein-protein interaction; resistance proteins, R proteins; soybean; transcription factor, TF; yeast two-hybrid, Y2H.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Dehydration
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Molecular Sequence Data
  • Phylogeny
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism
  • Protein Binding
  • Protein Interaction Mapping*
  • Soybeans / genetics
  • Soybeans / metabolism*
  • Stress, Physiological
  • Two-Hybrid System Techniques*


  • Plant Proteins