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, 182 (2), 197-9

Adaptation of the Nelson-Somogyi Reducing-Sugar Assay to a Microassay Using Microtiter Plates

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Adaptation of the Nelson-Somogyi Reducing-Sugar Assay to a Microassay Using Microtiter Plates

F Green 3rd et al. Anal Biochem.

Abstract

The Nelson-Somogyi assay for reducing sugars was adapted to microtiter plates. The primary advantages of this modified assay are (i) smaller sample and reagent volumes, (ii) elimination of boiling and filtration steps, (iii) automated measurement with a dual-wavelength scanning TLC densitometer, (iv) increased range and reproducibility, and (v) automated colorimetric readings by reflectance rather than absorbance.

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