ErmF and ereD are responsible for erythromycin resistance in Riemerella anatipestifer

PLoS One. 2015 Jun 24;10(6):e0131078. doi: 10.1371/journal.pone.0131078. eCollection 2015.

Abstract

To investigate the genetic basis of erythromycin resistance in Riemerella anatipestifer, the MIC to erythromycin of 79 R. anatipestifer isolates from China and one typed strain, ATCC11845, were evaluated. The results showed that 43 of 80 (53.8%) of the tested R. anatipestifer strains showed resistance to erythromycin, and 30 of 43 erythromycin-resistant R. anatipestifer strains carried ermF or ermFU with an MIC in the range of 32-2048 μg/ml, while the other 13 strains carrying the ereD gene exhibited an MIC of 4-16 μg/ml. Of 30 ermF + R. anatipestifer strains, 27 (90.0%) carried the ermFU gene which may have been derived from the CTnDOT-like element, while three other strains carried ermF from transposon Tn4351. Moreover, sequence analysis revealed that ermF, ermFU, and ereD were located within the multiresistance region of the R. anatipestifer genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Drug Resistance, Microbial / genetics*
  • Ducks
  • Erythromycin / pharmacology*
  • Escherichia coli
  • Genes, Bacterial
  • Methyltransferases / genetics*
  • Riemerella / drug effects
  • Riemerella / genetics*
  • Riemerella / isolation & purification

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Erythromycin
  • Methyltransferases
  • ErmF protein, Bacteroides

Grants and funding

This work was supported by the National Natural Science Foundation of China (31072156, 31272590 and 31472224). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.