Mechanical overloading causes mitochondrial superoxide and SOD2 imbalance in chondrocytes resulting in cartilage degeneration

Sci Rep. 2015 Jun 25;5:11722. doi: 10.1038/srep11722.

Abstract

Mechanical stress and aging are major risk factors of cartilage degeneration. Human studies have previously reported that oxidative damage increased, while SOD2 protein was reciprocally downregulated in osteoarthritic degenerated cartilage. However, it remains unclear whether mitochondrial superoxide imbalance in chondrocytes causes cartilage degeneration. We herein demonstrate that mechanical loading promoted mitochondrial superoxide generation and selective Sod2 downregulation in chondrocytes in vivo and that mitochondrial superoxide inducer also downregulated Sod2 expression in chondrocytes in vitro. A genetically manipulated model revealed that Sod2 deficiency in chondrocytes also resulted in mitochondrial superoxide overproduction and dysfunction, thus leading to cartilage degeneration. Intra-articular injection of a permeable antioxidant effectively suppressed the mechanical loading-induced mitochondrial superoxide generation and cartilage degeneration in mice. Our findings demonstrate that mitochondrial superoxide plays a pivotal role in the development and progression of osteoarthritis, and the mitochondrial superoxide balance may therefore be a promising target for the treatment of cartilage degeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology
  • Ascorbic Acid / administration & dosage
  • Ascorbic Acid / analogs & derivatives
  • Ascorbic Acid / pharmacology
  • Blotting, Western
  • Cartilage Diseases / genetics
  • Cartilage Diseases / metabolism*
  • Cartilage Diseases / prevention & control
  • Cartilage, Articular / drug effects
  • Cartilage, Articular / metabolism*
  • Cartilage, Articular / pathology
  • Cells, Cultured
  • Chondrocytes / drug effects
  • Chondrocytes / metabolism*
  • Chondrocytes / ultrastructure
  • Gene Expression / drug effects
  • Herbicides / pharmacology
  • Humans
  • Injections, Intra-Articular
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Microscopy, Electron
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Mitochondria / ultrastructure
  • Paraquat / pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stress, Mechanical
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism*
  • Superoxides / metabolism*
  • Weight-Bearing

Substances

  • Antioxidants
  • Herbicides
  • Superoxides
  • Superoxide Dismutase
  • superoxide dismutase 2
  • Paraquat
  • Ascorbic Acid