Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells

Nat Commun. 2015 Jun 25;6:7526. doi: 10.1038/ncomms8526.

Abstract

The immune response relies on the migration of leukocytes and on their ability to stop in precise anatomical locations to fulfil their task. How leukocyte migration and function are coordinated is unknown. Here we show that in immature dendritic cells, which patrol their environment by engulfing extracellular material, cell migration and antigen capture are antagonistic. This antagonism results from transient enrichment of myosin IIA at the cell front, which disrupts the back-to-front gradient of the motor protein, slowing down locomotion but promoting antigen capture. We further highlight that myosin IIA enrichment at the cell front requires the MHC class II-associated invariant chain (Ii). Thus, by controlling myosin IIA localization, Ii imposes on dendritic cells an intermittent antigen capture behaviour that might facilitate environment patrolling. We propose that the requirement for myosin II in both cell migration and specific cell functions may provide a general mechanism for their coordination in time and space.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens / metabolism*
  • Antigens, Differentiation, B-Lymphocyte / genetics
  • Antigens, Differentiation, B-Lymphocyte / metabolism*
  • Bone Marrow Cells
  • Cathepsins / genetics
  • Cathepsins / metabolism
  • Cell Movement / physiology*
  • Dendritic Cells / metabolism*
  • Female
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / metabolism*
  • Male
  • Mice
  • Microfluidic Analytical Techniques
  • Myosin Type II / genetics
  • Myosin Type II / metabolism*
  • Ovalbumin / metabolism*

Substances

  • Antigens
  • Antigens, Differentiation, B-Lymphocyte
  • Histocompatibility Antigens Class II
  • invariant chain
  • Ovalbumin
  • Cathepsins
  • cathepsin S
  • Myosin Type II