cir-ITCH plays an inhibitory role in colorectal cancer by regulating the Wnt/β-catenin pathway

Guanli Huang; Hua Zhu; Yixiong Shi; Wenzhi Wu; Huajie Cai; Xiangjian Chen

doi:10.1371/journal.pone.0131225

cir-ITCH plays an inhibitory role in colorectal cancer by regulating the Wnt/β-catenin pathway

PLoS One. 2015 Jun 25;10(6):e0131225. doi: 10.1371/journal.pone.0131225. eCollection 2015.

Authors

Guanli Huang¹, Hua Zhu², Yixiong Shi³, Wenzhi Wu³, Huajie Cai⁴, Xiangjian Chen⁴

Affiliations

¹ Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
² Departments of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
³ Department of gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
⁴ Department of endoscopic surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.

Abstract

Noncoding RNAs (ncRNAs) are the dominant product of eukaryotic transcription. These products range from short microRNAs (miRNAs) to long intergenic noncoding RNAs (lincRNAs). Circular RNAs composed of exonic sequences represent an understudied form of ncRNA that was discovered more than 20 years ago. Using a TaqMan-based reverse transcriptase polymerase chain reaction assay, we analyzed the relationship between cir-ITCH expression and colorectal cancer (CRC) in a total of 45 CRCs and paired adjacent non-tumor tissue samples. We found that cir-ITCH expression was typically down-regulated in CRC compared to the peritumoral tissue. This result, as well as several follow-up experiments, showed that cir-ITCH could increase the level of ITCH, which is involved in the inhibition of the Wnt/β-catenin pathway. Therefore, our results showed that cir-ITCH plays a role in CRC by regulating the Wnt/β-catenin pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
Adult
Binding Sites
Cell Survival
Colorectal Neoplasms / metabolism*
Dactinomycin / chemistry
Exoribonucleases / chemistry
Female
Gene Expression Regulation, Neoplastic*
HCT116 Cells
Humans
Male
MicroRNAs / metabolism*
Middle Aged
Plasmids / metabolism
RNA / metabolism*
RNA, Circular
RNA, Untranslated / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Wnt Proteins / metabolism
Wnt Signaling Pathway*
beta Catenin / metabolism

Substances

3' Untranslated Regions
MicroRNAs
RNA, Circular
RNA, Untranslated
Wnt Proteins
beta Catenin
cir-ITCH noncoding RNA, human
Dactinomycin
RNA
Exoribonucleases
ribonuclease R

Grants and funding

This work was supported by a grant from Wenzhou Science and Technology Bureau Natural Science Foundation (Y20140700 Dr. XC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.