Quantitative analysis of the human T cell palmitome

Sci Rep. 2015 Jun 26;5:11598. doi: 10.1038/srep11598.

Abstract

Palmitoylation is a reversible post-translational modification used to inducibly compartmentalize proteins in cellular membranes, affecting the function of receptors and intracellular signaling proteins. The identification of protein "palmitomes" in several cell lines raises the question to what extent this modification is conserved in primary cells. Here we use primary T cells with acyl-biotin exchange and quantitative mass spectrometry to identify a pool of proteins previously unreported as palmitoylated in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Cells, Cultured
  • Chromatography, Liquid
  • Humans
  • Jurkat Cells
  • Lipoylation
  • Protein Processing, Post-Translational*
  • Proteome / metabolism*
  • Proteomics / methods*
  • T-Lymphocytes / metabolism*
  • Tandem Mass Spectrometry

Substances

  • Proteome