The WUSCHEL-RELATED HOMEOBOX 3 gene PaWOX3 regulates lateral organ formation in Norway spruce

New Phytol. 2015 Dec;208(4):1078-88. doi: 10.1111/nph.13536. Epub 2015 Jun 25.

Abstract

In angiosperms, WUSCHEL-RELATED HOMEOBOX 3 (WOX3) genes are required for the recruitment of founder cells from the lateral domains of shoot meristems that form lateral regions of leaves. However, the regulation of the formation of lateral organs in gymnosperms remains unknown. By using somatic embryos of Norway spruce (Picea abies) we have studied the expression and function of PaWOX3 during embryo development. The mRNA abundance of PaWOX3 was determined by quantitative real-time PCR, and the spatial expression of PaWOX3 was analysed by histochemical β-glucuronidase (GUS) assays and in situ mRNA hybridization. To investigate the function of PaWOX3, we analysed how downregulation of PaWOX3 in RNA interference lines affected embryo development and morphology. PaWOX3 was highly expressed in mature embryos at the base of each cotyledon close to the junction between the cotyledons, and in the lateral margins of cotyledons and needles, separating them into an adaxial and an abaxial side. Downregulation of the expression of PaWOX3 caused defects in lateral margin outgrowth in cotyledons and needles, and reduced root elongation. Our data suggest that the WOX3 function in margin outgrowth in lateral organs is conserved among the seed plants, whereas its function in root elongation may be unique to gymnosperms.

Keywords: Norway spruce (Picea abies); WUSCHEL-RELATED HOMEOBOX; cotyledon; embryogenesis; lateral organ; needle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cotyledon / growth & development
  • Gene Expression Regulation, Developmental*
  • Gene Expression Regulation, Plant
  • Genes, Homeobox*
  • Genes, Plant*
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism
  • Organogenesis, Plant / genetics*
  • Picea / genetics*
  • Picea / growth & development
  • Picea / metabolism
  • Plant Leaves / growth & development
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Roots / growth & development
  • Plant Shoots / growth & development*
  • RNA, Messenger / metabolism
  • Seeds / growth & development

Substances

  • Homeodomain Proteins
  • Plant Proteins
  • RNA, Messenger