Catecholaminergic projections to tuberoinfundibular neurones of the paraventricular nucleus: I. Effects of stimulation of A1, A2, A6 and C2 cell groups

Abstract

Extracellular electrical activity was recorded from 203 paraventricular nucleus (PVN) neurones antidromically identified as projecting to the median eminence. Spontaneous activity and the effects of stimulation of the A1, A2, A6 and C2 catecholaminergic cell groups upon the PVN neurones were examined. Cells were located at a mean height 2.29 +/- 0.03 mm above the base of the brain, corresponding with the corticotropin-releasing factor (CRF) rich component of the nucleus. The mean firing rate was 3.2 +/- 0.3 Hz and antidromic invasion latency was 9.9 +/- 0.3 msec. Seventy-six % of cells tested were activated by painful somatosensory stimuli. Electrical stimulation of the A1 or A2 region evoked excitatory responses from the majority of cells tested (76% and 85%, respectively), whilst stimulation of the A6 and C2 regions evoked more inhibitory responses (43% and 59%, respectively). Most responses (56%), whether excitatory or inhibitory, were not clearly defined in terms of latency, and were only observed following delivery of 5-10 single shocks at 0.5 Hz. Excitation recorded following A1 and A2 stimulation suggests a facilitatory role for noradrenaline in the regulation of PVN activity. Inhibitory responses following C2 stimulation indicate that adrenaline may serve to inhibit such activity, whilst the more mixed responses following A6 stimulation suggest that the projections of this region differ in some way from those of the A1 and A2 cells. Response reversals were observed, after delivery of higher frequency stimulation, for a substantial proportion (20%) of the cells tested.