Silk fibroin/sodium alginate composite nano-fibrous scaffold prepared through thermally induced phase-separation (TIPS) method for biomedical applications

Mater Sci Eng C Mater Biol Appl. 2015 Oct:55:8-13. doi: 10.1016/j.msec.2015.05.052. Epub 2015 May 21.

Abstract

To mimic the natural fibrous structure of the tissue extracellular matrix, a nano-fibrous silk fibroin (SF)/sodium alginate (SA) composite scaffold was fabricated by a thermally-induced phase-separation method. The effects of SF/SA ratio on the structure and the porosity of the composite scaffolds were examined. Scanning electron microscopy and porosity results showed that the 5SF/1SA and 3SF/1SA scaffolds possessed an excellent nano-fibrous structure and a porosity of more than 90%. Fourier transform infrared, X-ray diffraction, and differential scanning calorimetry results indicated the physical interaction between SF and SA molecules and their good compatibility in the 5SF/1SA and 3SF/1SA scaffolds, whereas they showed less compatibility in the 1SF/1SA scaffold. Cell culture results showed that MG-63 cells can attach and grow well on the surface of the SF/SA scaffolds. The nano-fibrous SF/SA scaffold can be potentially used in tissue engineering.

Keywords: Cell biocompatibility; Nano-fibrous; Silk fibroin; Sodium alginate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Compounds / chemistry*
  • Animals
  • Biocompatible Materials / chemistry*
  • Bombyx / chemistry
  • Calorimetry, Differential Scanning / methods
  • Cell Line
  • Fibroins / chemistry*
  • Humans
  • Materials Testing / methods
  • Microscopy, Electron, Scanning / methods
  • Nanofibers / chemistry*
  • Porosity
  • Silk / chemistry*
  • Sodium Compounds / chemistry*
  • Tissue Engineering / methods
  • Tissue Scaffolds / chemistry*
  • X-Ray Diffraction / methods

Substances

  • Aluminum Compounds
  • Biocompatible Materials
  • Silk
  • Sodium Compounds
  • sodium aluminum hydride
  • Fibroins