McKeever (1979) reported that left handers who wrote with an inverted handwriting posture (IHP) had a higher incidence of left handedness in their families than did left handers who wrote with a non-inverted handwriting posture (NHP). Parlow and Kinsbourne (1981), however, failed to find a relationship between handwriting posture (HWP) and positive familial sinistrality (FS+). McKeever had classified his left handed subjects according to whether they had several, one, or no left handers among their first degree relatives and biologically-related second degree relatives. Parlow and Kinsbourne classified subjects as FS- or FS+ on the basis of first degree relatives and did not inquire about the handedness of second degree relatives. In the present study, handedness for writing of all first and second degree relatives was recorded for 216 NIP and 255 IHP left handers. Results showed a significant association of HWP and FS factors when second degree relatives were considered, but not when second degree relative handedness was ignored. The relationship was strongest within the maternal family line, where the proportion of left handers was more than twice as high for the IHP as for the NHP group. These findings suggest that HWP may well be of some importance for a complete understanding of the cerebral organization of left handers.