Immunohistochemistry of lymphocytes in benign lymphoadenosis of oral mucosa

Genet Mol Res. 2015 Jun 29;14(2):7163-71. doi: 10.4238/2015.June.29.10.


Benign lymphoadenosis of oral mucosa (BLOM) is a common oral mucosa disease and may be regarded as a precancerous lesion. However, the association between its biological behavior and lymphocyte distribution remains unclear. Therefore, to investigate the characteristics of BLOM, we studied the infiltration of lymphocytes associated with it. The expression levels of CD74, CD20, CD3, and CD45RO were evaluated by immunohistochemical staining in 14 sam-ples from BLOM, 9 samples from BLOM with atypia hyperplasia, 11 samples from BLOM with canceration, and 10 samples from normal oral mucosa tissues. The results were analyzed by two-sample t-test using SPSS 10.0 for Windows, and P < 0.05 was considered to be sig-nificant. In normal oral mucosa, positive expression levels of CD3 and CD45RO were presented in the extra-lymphoid follicle, and the expres-sion levels of CD74 and CD20 were negative. In all BLOM groups, the expression level of CD20 was positive except for one case of BLOM with canceration; the expression levels of CD74 were all positive. Posi-tive expression levels of CD3 and CD45RO could be found not only in extra-lymphoid follicles but also in inner-lymphoid follicles in the BLOM groups. The expression levels of CD74 and CD20 in extra-lym-phoid follicles, and CD3 and CD45RO in inner-lymphoid follicles in BLOM were significantly higher than in BLOM with canceration. The infiltrated lymphocytes in BLOM comprise T- and B-cells. This indi-cates that the lymphoid tissue in BLOM is mucosa-associated lymphoid tissue and BLOM is a proliferative lesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD20 / genetics
  • Antigens, CD20 / immunology
  • Antigens, Differentiation, B-Lymphocyte / genetics
  • Antigens, Differentiation, B-Lymphocyte / immunology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / pathology
  • CD3 Complex / genetics
  • CD3 Complex / immunology
  • Gene Expression / immunology*
  • Histocompatibility Antigens Class II / genetics
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Immunohistochemistry
  • Leukocyte Common Antigens / genetics
  • Leukocyte Common Antigens / immunology
  • Lymphoid Tissue / immunology
  • Lymphoid Tissue / pathology
  • Lymphoma, B-Cell, Marginal Zone / genetics
  • Lymphoma, B-Cell, Marginal Zone / immunology*
  • Lymphoma, B-Cell, Marginal Zone / pathology
  • Mouth Mucosa / immunology*
  • Mouth Mucosa / pathology
  • Neoplasms / genetics
  • Neoplasms / immunology*
  • Neoplasms / pathology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / pathology


  • Antigens, CD20
  • Antigens, Differentiation, B-Lymphocyte
  • CD3 Complex
  • Histocompatibility Antigens Class II
  • invariant chain
  • Leukocyte Common Antigens