NMR assignments of the C-terminal domain of human galectin-8

Chun-Hao Gerard Liu; Chih-Ta Henry Chien; Chun-Hung Lin; Shang-Te Danny Hsu

doi:10.1007/s12104-015-9623-1

NMR assignments of the C-terminal domain of human galectin-8

Biomol NMR Assign. 2015 Oct;9(2):427-30. doi: 10.1007/s12104-015-9623-1. Epub 2015 Jul 1.

Authors

Chun-Hao Gerard Liu^{1

2

3}, Chih-Ta Henry Chien^{1

4}, Chun-Hung Lin^{1

4

5}, Shang-Te Danny Hsu^{6

7}

Affiliations

¹ Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan.
² Institue of Bioinformatics and Structural Biology, National Tsin Hua University, Hsinchu, 30013, Taiwan.
³ Chemical Biology and Molecular Biophysics Program, Taiwan International Graduate Program, Academia Sinica, Nankang, Taipei, 11529, Taiwan.
⁴ Department of Chemistry, National Taiwan University, Taipei, 106, Taiwan.
⁵ Institute of Biochemical Science, National Taiwan University, Taipei, 106, Taiwan.
⁶ Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan. sthsu@gate.sinica.edu.tw.
⁷ Institute of Biochemical Science, National Taiwan University, Taipei, 106, Taiwan. sthsu@gate.sinica.edu.tw.

PMID: 26126590
DOI: 10.1007/s12104-015-9623-1

Abstract

Galectins recognize β-galectosides to promote a variety of cellular functions. Despite their sequence variations, all galectins share the same carbohydrate recognition domains (CRD) and their modes of ligand recognition at a structural level are essentially identical. Human galectin 8 plays an important role in numerous cancer and immune responses. It consists of two CRDs that are connected via a flexible linker. The substrate affinities and specificities of the N- and C-terminal domains are quite different. In order to investigate the structural basis of their substrate specificities, we complete the NMR (1)H, (13)C, and (15)N chemical shift assignments of C-terminal domain of human galectin-8 (hG8C).

Keywords: Carbohydrate recognition domain; Galectin 8; NMR spectroscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Galectins / chemistry*
Humans
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular*
Protein Structure, Secondary
Protein Structure, Tertiary

Substances

Galectins
LGALS8 protein, human