ANP/NPRA signaling preferentially mediates Th2 responses in favor of pathological processes during the course of acute allergic asthma

Libing Ma; Jinrong Zeng; Biwen Mo; Changming Wang; Yabing Sun; Meng Zhang; Shaokun Liu; Xudong Xiang; Cong-Yi Wang

ANP/NPRA signaling preferentially mediates Th2 responses in favor of pathological processes during the course of acute allergic asthma

Int J Clin Exp Med. 2015 Apr 15;8(4):5121-8. eCollection 2015.

Authors

Libing Ma¹, Jinrong Zeng¹, Biwen Mo¹, Changming Wang¹, Yabing Sun¹, Meng Zhang², Shaokun Liu³, Xudong Xiang⁴, Cong-Yi Wang⁵

Affiliations

¹ Department of Respiratory Medicine, The Affiliated Hospital of Guilin Medical University Guilin 541001, China ; Institute of Respiratory Diseases, Guilin Medical University Guilin 541001, China ; Key Laboratory of Respiratory Diseases of Colleges and Universities Affiliated Education Department of Guangxi Zhuang Autonomous Region Guilin 541001, China.
² The Center for Biomedical Research, Tongji Hospital, Huazhong University of Science and Technology Wuhan 430030, China.
³ Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, China.
⁴ Institute of Emergency Medicine and Rare Diseases, Central South University Changsha 410011, China ; Department of Emergency Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, China.
⁵ Institute of Emergency Medicine and Rare Diseases, Central South University Changsha 410011, China ; Department of Emergency Medicine, The Second Xiangya Hospital, Central South University Changsha 410011, China ; The Center for Biomedical Research, Tongji Hospital, Huazhong University of Science and Technology Wuhan 430030, China.

PMID: 26131085
PMCID: PMC4483987

Abstract

Although atrial natriuretic peptide (ANP) has been well recognized for its role in the regulation of volume-pressure homeostasis in cardiovascular system, its impact on respiratory system, particularly on the pathogenesis of acute allergic asthma, is yet to be elucidated. In the present report, we induced mice with OVA for onset of acute allergic asthma along with the administration of recombinant ANP or A71915 (an antagonist for ANP/natriuretic peptide receptor A, NPRA). It was noted that treatment of mice with ANP significantly promoted inflammatory infiltration in the airway and the production of inflammatory cytokines in the bronchoalveolar lavage fluid (BALF) and lung homogenates, and the number of inflammatory cells in the BALF was significantly higher as compared with that of PBS treated asthmatic mice. Moreover, blockade of ANP/NPRA signaling by A71915 almost completely attenuated the effect of ANP administration. Mechanistic studies revealed that ANP repressed the expression of Th1 transcription factor T-bet, but enhanced Th2 transcription GATA3 expression. Together, our data provided feasible evidence suggesting that ANP/NPRA signaling predominantly induces a Th2-type response in favor of pathological processes during the course of acute allergic asthma.

Keywords: Atrial natriuretic peptide/Natriuretic peptide receptor A (ANP/NPRA) signaling; Th1/Th2 cells; acute allergic asthma; airway inflammation.