Proteomic profiling of posterior longitudinal ligament of cervical spine

Ying Zhang; Baifeng Liu; Jiang Shao; Jia Song; Jing Zhang

Proteomic profiling of posterior longitudinal ligament of cervical spine

Int J Clin Exp Med. 2015 Apr 15;8(4):5631-9. eCollection 2015.

Authors

Ying Zhang¹, Baifeng Liu², Jiang Shao³, Jia Song³, Jing Zhang³

Affiliations

¹ Department of Orthopedics, Changzheng Hospital Shanghai 200003, China.
² Department of Orthopedics, Daqing Peopele's Hospital Daqing 163316, Heilongjiang Province, China.
³ Department of Orthopedic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine 1665 Kongjiang Road, Shanghai 200092, China.

PMID: 26131146
PMCID: PMC4483833

Abstract

Objective: To identify putative biomarkers for ossification of posterior longitudinal ligament (OPLL).

Material and methods: Proteomic analysis was performed in 4 ligament samples from OPLL patients and healthy controls. RT-PCR was used to further verify the proteomic analysis results.

Results: A total of 50 differentially expressed spots were detected in 2-D electrophoresis between the two groups. In protein/peptide analysis, 21 proteins or peptides were finally identified. Besides 13 hematic proteins and 2 unknown proteins, 6 other proteins were differentially expressed. Among them, carbonic anhydrase I, NAD(P) dependent steroid dehydrogenase-like, billiverdin reductase B and alpha-1 collagen VI were down-regulated, while osteoglycin and nebulin-related anchoring protein were up-regulated. The results of NAD(P) dependent steroid dehydrogenase-like, alpha-1 collagen VI and nebulin-related anchoring protein were validated by RT-PCR.

Conclusion: These differentially expressed proteins could play a role in the onset and progression of OPLL.

Keywords: 2-dimensionl electrophoresis; Ossification of posterior longitudinal ligament; biomarker; diagnosis.