Abstract
The effect of para vs meta substitution on the biological behavior of an intact antibody and an F(ab')2 fragment was investigated. Paired-label studies were performed using 81C6 IgG and OC 125 F(ab')2 labeled using the N-succinimidyl esters of both p-[125I]- and m-[131I]iodobenzoate as well as with the potential catabolites, p-[125I]- and m-[131I]iodobenzoic acid. In all 3 studies, up to 55% lower uptake of 131I in thyroid and stomach was observed, suggesting that the m-substituted species were more inert to dehalogenation in vivo.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal / pharmacokinetics
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Drug Stability
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Immunoglobulin Fab Fragments / immunology
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Immunoglobulin Fab Fragments / pharmacokinetics
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Iodine Radioisotopes
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Iodobenzoates / chemical synthesis*
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Iodobenzoates / pharmacokinetics
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Isotope Labeling / methods*
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Male
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Mice
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Mice, Inbred BALB C
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Neoplasm Proteins / immunology
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Tissue Distribution
Substances
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Antibodies, Monoclonal
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Immunoglobulin Fab Fragments
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Iodine Radioisotopes
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Iodobenzoates
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Neoplasm Proteins
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N-succinimidyl 4-iodobenzoate
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N-succinimidyl 3-iodobenzoate