Development of a multimodal imaging probe by encapsulating iron oxide nanoparticles with functionalized amphiphiles for lymph node imaging

Nanomedicine (Lond). 2015 Jul;10(12):1899-910. doi: 10.2217/nnm.15.41.

Abstract

Aim: We tried to develop a multimodal iron oxide nanoparticles (IO NP) imaging probe by an encapsulation method using specific amphiphiles for (68)Ga-labeling and lymph node-targeting.

Materials & methods: Nanoparticles (NPs) were encapsulated with a solution containing polysorbate 60 and the amphiphiles. The prepared NPs were labeled with (68)Ga and tested in vitro and in vivo.

Results: Prepared 1,4,7-triazacyclononane-1,4,7-triacetic acid-IO-Mannose (NOTA-IO-Man) showed a narrow size distribution, and no significant aggregation or degradation under harsh conditions. The relaxivity coefficient of (68)Ga-NOTA-IO-Man was higher than that of ferumoxide. The accumulation of (68)Ga-NOTA-IO-Man in the lymph node after injection into rat's footpad was confirmed by both positron emission tomography and MRI.

Conclusion: We successfully developed PET/MRI dual-modality imaging probe targeting lymph nodes by using the facile encapsulation method.

Keywords: 68Ga; PET/MRI; encapsulation; iron oxide nanoparticles; tween60.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Contrast Media / chemistry
  • Gallium Radioisotopes / chemistry
  • Lymph Nodes / cytology*
  • Lymph Nodes / diagnostic imaging*
  • Magnetic Resonance Imaging / methods*
  • Magnetite Nanoparticles / chemistry*
  • Magnetite Nanoparticles / ultrastructure
  • Male
  • Multimodal Imaging / methods
  • Nanocapsules / chemistry*
  • Nanocapsules / ultrastructure
  • Particle Size
  • Positron-Emission Tomography / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Surface-Active Agents / chemical synthesis

Substances

  • Contrast Media
  • Gallium Radioisotopes
  • Magnetite Nanoparticles
  • Nanocapsules
  • Surface-Active Agents