Contribution of 3-hydroxyisobutyrate to the measurement of 3-hydroxybutyrate in human plasma: comparison of enzymatic and gas-liquid chromatography-mass spectrometry assays in normal and in diabetic subjects

J Lipid Res. 1989 Nov;30(11):1811-7.


In this study we employed a capillary gas-liquid chromatographic-mass spectrometric (GLC-MS) method to measure the plasma concentrations of 3-hydroxybutyrate (3-OHB) and 3-hydroxyisobutyrate (3-OHIB) in overnight fasted diabetic subjects and in normal subjects. Plasma contents of 3-hydroxybutyrate measured in this fashion were identical to those obtained by enzymatic assay using a commercial preparation of beta-hydroxybutyrate dehydrogenase, indicating no significant contamination of this enzyme preparation with 3-hydroxyisobutyrate dehydrogenase. In normal individuals, plasma 3-OHIB concentration was 21 +/- 2 microM in the overnight fasted state and was higher in diabetic subjects (38 +/- 5 microM) and in subjects fasted for 72 h (97 +/- 4 microM). In the postabsorptive state, 3-OHIB was 33% the concentration of 3-OHB in normals and 17% that of 3-OHB in the diabetics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Hydroxybutyric Acid
  • Adult
  • Diabetes Mellitus / blood*
  • False Positive Reactions
  • Fasting
  • Fluorometry
  • Food
  • Gas Chromatography-Mass Spectrometry*
  • Humans
  • Hydroxybutyrate Dehydrogenase*
  • Hydroxybutyrates / blood*
  • Quality Control


  • Hydroxybutyrates
  • Hydroxybutyrate Dehydrogenase
  • 3-hydroxyisobutyric acid
  • 3-Hydroxybutyric Acid