Short-chain Polyisoprenoids in the Yeast Saccharomyces Cerevisiae - New Companions of the Old Guys

Biochim Biophys Acta. 2015 Oct;1851(10):1296-303. doi: 10.1016/j.bbalip.2015.06.006. Epub 2015 Jul 2.

Abstract

Dolichols are, among others, obligatory cofactors of protein glycosylation in eukaryotic cells. It is well known that yeast cells accumulate a family of dolichols with Dol-15/16 dominating while upon certain physiological conditions a second family with Dol-21 dominating is noted. In this report we identified the presence of additional short-chain length polyprenols - all-trans Pren-7 in three yeast strains (SS328, BY4741 and L5366), Pren-7 was accompanied by traces of putative Pren-6 and -8. Moreover, in two of these strains a single polyprenol mainly-cis-Pren-11 was synthesized at the stationary phase of growth. Identity of polyprenols was confirmed by HR-HPLC/MS, NMR and metabolic labeling. Additionally, simvastatin inhibited their biosynthesis.

Keywords: Dolichol; Polyisoprenoid alcohols; Prenol; Saccharomyces cerevisiae; yeast.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Terpenes / metabolism*

Substances

  • Terpenes