Intron-based genomic editing: a highly efficient method for generating knockin zebrafish

Oncotarget. 2015 Jul 20;6(20):17891-4. doi: 10.18632/oncotarget.4547.

Abstract

The TALEN and CRISPR/Cas9 nuclease systems have been extensively utilized in genomic engineering of model organisms. In zebrafish, the nuclease systems have been successfully applied in generating loss-of-function knockout lines. However, genome-specific knockin techniques in zebrafish are still at the beginning. In this perspective, we briefly summarize the recent progresses on knockin approaches in zebrafish with a special focus on the newly developed intron-based knockin method.

Keywords: CRISPR/Cas9; knockin; zebrafish.

Publication types

  • Review

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • DNA End-Joining Repair
  • Gene Expression Regulation
  • Gene Knock-In Techniques*
  • Genes, Reporter
  • Genotype
  • Introns*
  • Phenotype
  • Recombinational DNA Repair
  • Zebrafish / genetics*
  • Zebrafish / metabolism