Physical and genetic mapping of the protein A gene in the chromosome of Staphylococcus aureus 8325-4

J Gen Microbiol. 1989 Jul;135(7):1799-807. doi: 10.1099/00221287-135-7-1799.

Abstract

The gene coding for protein A (spa) has been mapped close to nov on the genetic map of the chromosome of Staphylococcus aureus 8325-4. A rapid mapping procedure has been developed which first allowed the region of the chromosome carrying the spa gene to be identified by blot +hybridization of large DNA fragments which had been separated by pulsed-field gel electrophoresis. Restriction endonuclease SmaI fragment G was shown to carry the spa gene. An insertion mutation in spa was constructed by in vitro insertion of a fragment of DNA expressing resistance to kanamycin and neomycin. A spa::Kan(r)Neo(r) mutation was isolated in S. aureus 8325-4 by allele replacement. This provided a selectable marker which allowed the spa gene to be mapped by transformation analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Chromosome Mapping
  • Chromosomes, Bacterial
  • DNA, Bacterial / genetics
  • Genes, Bacterial*
  • Recombinant Proteins / genetics
  • Staphylococcal Protein A / genetics*
  • Staphylococcus aureus / genetics*
  • Staphylococcus aureus / pathogenicity
  • Transformation, Bacterial
  • Virulence

Substances

  • DNA, Bacterial
  • Recombinant Proteins
  • Staphylococcal Protein A