Manipulating the in vivo immune response by targeted gene knockdown

Curr Opin Immunol. 2015 Aug;35:63-72. doi: 10.1016/j.coi.2015.06.005. Epub 2015 Jul 3.


Aptamers, nucleic acids selected for high affinity binding to proteins, can be used to activate or antagonize immune mediators or receptors in a location and cell-type specific manner and to enhance antigen presentation. They can also be linked to other molecules (other aptamers, siRNAs or miRNAs, proteins, toxins) to produce multifunctional compounds for targeted immune modulation in vivo. Aptamer-siRNA chimeras (AsiCs) that induce efficient cell-specific knockdown in immune cells in vitro and in vivo can be used as an immunological research tool or potentially as an immunomodulating therapeutic.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Aptamers, Nucleotide / chemical synthesis
  • Aptamers, Nucleotide / therapeutic use*
  • Gene Knockdown Techniques
  • Humans
  • Immunomodulation
  • Immunotherapy / methods*
  • Molecular Targeted Therapy*
  • RNA, Small Interfering / chemical synthesis
  • RNA, Small Interfering / therapeutic use*


  • Aptamers, Nucleotide
  • RNA, Small Interfering