Comparative expression analysis of cysteine-rich intestinal protein family members crip1, 2 and 3 during Xenopus laevis embryogenesis

Int J Dev Biol. 2014;58(10-12):841-9. doi: 10.1387/ijdb.140270sk.

Abstract

Members of the cysteine-rich intestinal protein (Crip) family belong to the group 2 LIM proteins. Crip proteins are widely expressed in adult mammals but their expression profile and function during embryonic development are still mostly unknown. In this study, we have described for the first time the spatio-temporal expression pattern of the three family members crip1, crip2 and crip3 during Xenopus laevis embryogenesis by RT-PCR and whole mount in situ hybridization approaches. We observed that all three genes are expressed in the pronephros, branchial arches and the eye. Furthermore, crip1 transcripts could be visualized in the developing cranial ganglia and neural tube. In contrast, crip2 could be detected in the cardiovascular system, the brain and the neural tube while crip3 was expressed in the cranial ganglions and the heart. Based on these findings, we suggest that each crip family member may play an important role during embryonic development.

Publication types

  • Comparative Study

MeSH terms

  • Adaptor Proteins, Signal Transducing / biosynthesis*
  • Adaptor Proteins, Signal Transducing / genetics
  • Animals
  • Brain / metabolism
  • Branchial Region / metabolism
  • Cardiovascular System / metabolism
  • Embryo, Nonmammalian / metabolism
  • Embryonic Development
  • Eye / metabolism
  • Ganglia / metabolism
  • Gene Expression Regulation, Developmental / genetics*
  • In Situ Hybridization
  • LIM Domain Proteins / biosynthesis*
  • LIM Domain Proteins / genetics
  • Myocardium / metabolism
  • Neural Tube / metabolism
  • Pronephros / metabolism
  • Xenopus Proteins / biosynthesis*
  • Xenopus Proteins / genetics
  • Xenopus laevis

Substances

  • Adaptor Proteins, Signal Transducing
  • CRIP1 protein, Xenopus laevis
  • CRIP2 protein, Xenopus laevis
  • CRIP3 protein, Xenopus laevis
  • LIM Domain Proteins
  • Xenopus Proteins