Cloning and sequence analysis of the 18 S ribosomal RNA gene of tomato and a secondary structure model for the 18 S rRNA of angiosperms

Mol Gen Genet. 1989 Oct;219(1-2):17-25. doi: 10.1007/BF00261152.

Abstract

The gene of a cytoplasmic 18 S ribosomal RNA (18 S rDNA) of the dicotyledonous plant tomato (Lycopersicon esculentum) cv. Rentita has been cloned, and its complete primary structure has been determined. The tomato 18 S rDNA is 1805 bp long with a G + C content of 49.6%. Its sequence exhibits 94%-96% positional identity when it is colinearly aligned with the previously reported sequences of the 17-18 S rDNAs of the dicot soybean and the monocots maize and rice. A model of the secondary structure of the 18 S rRNA of angiosperms is presented and its genera-specific structural features are compared with a current eukaryotic 18 S rRNA consensus model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Plants / genetics*
  • RNA, Ribosomal / genetics*
  • RNA, Ribosomal, 18S / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • RNA, Ribosomal
  • RNA, Ribosomal, 18S