[Effects of Blending Oil of Lavender and Thyme on Oxidative Stress, Immunity, and Skin Condition in Atopic Dermatitis Induced Mice]

J Korean Acad Nurs. 2015 Jun;45(3):367-77. doi: 10.4040/jkan.2015.45.3.367.
[Article in Korean]


Purpose: The purpose of this study was to evaluate the effects of essential oil on oxidative stress, immunity, and skin condition in atopic dermatitis (AD) induced mice.

Methods: This study was a 3×3 factorial design. Factors were oil type (Lavender, Thyme, and 2:1 mixture of lavender and thyme oil [blending oil]) and treatment period (0 day, 7 days, and 21 days). The samples were 45 mice with AD and randomly assigned to nine groups of five mice per group. The dependent variables such as superoxide radical, IgE, degranulated mast cells, and epidermal thickness were measured. Data were collected from February to April in 2014. Descriptive statistics, One-way ANOVA, Two-way ANOVA, and Tukey's HSD test were performed using the SPSS WIN 20.0 program.

Results: Dependent variables were not statistically significantly different by the three oil types (p>.05). Essential oils such as lavender, thyme, and blending oil were all effective in reducing AD symptoms and especially 2:1 blending oil were most effective. There were statistically significant differences by the three treatment periods in all dependent variables (p<.001). There were statistically significant interactions between oil types and treatment periods in all dependent variables (p<.01). For decreasing superoxide radical, degranulated mast cells, and epidermal thickness, 2:1 mixed oil should be applied for at least 21 days. Otherwise to reduce IgE, 2:1 mixed oil should be used for at least 7 days.

Conclusion: These findings provide bases for developing effective interventions for AD patients to manage their AD symptoms.

Keywords: Atopic dermatitis; Essential oil; IgE; Mast cell; Superoxide radical.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Dermatitis, Atopic / chemically induced
  • Dermatitis, Atopic / drug therapy*
  • Dermatitis, Atopic / pathology
  • Disease Models, Animal
  • Immunity* / drug effects
  • Immunoglobulin E / blood
  • Lavandula / chemistry*
  • Lavandula / metabolism
  • Mast Cells / cytology
  • Mast Cells / metabolism
  • Mice
  • Oils, Volatile / chemistry
  • Oils, Volatile / pharmacology
  • Oils, Volatile / therapeutic use
  • Oxidative Stress* / drug effects
  • Picryl Chloride / toxicity
  • Plant Oils / chemistry
  • Plant Oils / pharmacology
  • Plant Oils / therapeutic use*
  • Singlet Oxygen / metabolism
  • Skin / drug effects
  • Skin / pathology
  • Thymus Plant / chemistry*
  • Thymus Plant / metabolism


  • Oils, Volatile
  • Plant Oils
  • Singlet Oxygen
  • Immunoglobulin E
  • Picryl Chloride