Enamel Deproteinization using Papacarie and 10% Papain Gel on Shear Bond Strength of Orthodontic Brackets Before and After Acid Etching

J Clin Pediatr Dent. 2015 Summer;39(4):348-57. doi: 10.17796/1053-4628-39.4.348.

Abstract

Objective: To suggest Papacarie(®) as a new deproteinizing agent in comparison with indigenously prepared 10% papain gel before and after acid etching that may enhance the quality of the bond between enamel surface and composite resin complex.

Study design: One hundred and twenty five extracted human premolars were utilized and divided into five groups: In the group 1, enamel surface was etched and primer was applied. In group 2, treatment with papacarie(®) for 60 seconds followed by etching and primer application. In group 3, etching followed by treatment with papacarie(®) for 60 seconds and primer application. In group 4, treatment with 10% papain gel for 60 seconds followed by etching and primer application. In group 5, etching followed by treatment with 10% papain gel for 60 seconds and primer application . After bonding the brackets, the mechanical testing was performed using a Universal testing machine. The failure mode was analyzed using an adhesive remnant index. The etching patterns before and after application of papacarie(®) and 10% papain gel was also evaluated using SEM. The values obtained for shear bond strength were submitted to analysis of variance and Tukey test (p < 0.05).

Results: It was observed that group 2 and group 4 had the highest shear bond strength and was statistically significant from other groups (p=0.001). Regarding Adhesive remnant index no statistical difference was seen between the groups (p=0.538).

Conclusion: Papacarie(®) or 10% papain gel can be used to deproteinize the enamel surface before acid etching to enhance the bond strength of orthodontic brackets.

Keywords: Enamel deproteinization; Etching; Papacarie®; Papain gel; Shear bond strength.

MeSH terms

  • Acid Etching, Dental / methods*
  • Adhesiveness
  • Dental Bonding / methods*
  • Dental Enamel / drug effects*
  • Dental Enamel / ultrastructure
  • Dental Enamel Proteins / drug effects*
  • Dental Stress Analysis / instrumentation
  • Gels
  • Humans
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Orthodontic Brackets*
  • Papain / chemistry
  • Papain / therapeutic use*
  • Phosphoric Acids / chemistry
  • Protein Denaturation
  • Resin Cements / chemistry
  • Shear Strength
  • Stress, Mechanical
  • Surface Properties

Substances

  • Dental Enamel Proteins
  • Gels
  • Papacarie
  • Phosphoric Acids
  • Resin Cements
  • Transbond XT
  • phosphoric acid
  • Papain