A ratiometric fluorescent system for carboxylesterase detection with AIE dots as FRET donors

Yinglong Wu; Shuailing Huang; Fang Zeng; Jun Wang; Changmin Yu; Jing Huang; Huiting Xie; Shuizhu Wu

doi:10.1039/c5cc04771d

A ratiometric fluorescent system for carboxylesterase detection with AIE dots as FRET donors

Chem Commun (Camb). 2015 Aug 18;51(64):12791-4. doi: 10.1039/c5cc04771d.

Authors

Yinglong Wu¹, Shuailing Huang, Fang Zeng, Jun Wang, Changmin Yu, Jing Huang, Huiting Xie, Shuizhu Wu

Affiliation

¹ College of Materials Science and Engineering, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou 510640, P. R. China. mcfzeng@scut.edu.cn shzhwu@scut.edu.cn.

PMID: 26165151
DOI: 10.1039/c5cc04771d

Abstract

A ratiometric fluorescent system for CaE detection with AIE dots as the FRET donors was designed. Upon enzymatic reaction, electrostatic interaction between the cationic TPE-N(+) dots and the enzymatic reaction product - the negatively charged fluorescein molecules - allows the FRET process to proceed, thus affording the ratiometric fluorescence CaE assay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carboxylic Ester Hydrolases / chemistry
Carboxylic Ester Hydrolases / metabolism*
Enzyme Assays / methods*
Fluorescein / chemistry
Fluorescence Resonance Energy Transfer / methods*
Fluorescent Dyes / chemistry
Static Electricity

Substances

Fluorescent Dyes
Carboxylic Ester Hydrolases
Fluorescein