Defensive Armor of Potato Tubers: Nonpolar Metabolite Profiling, Antioxidant Assessment, and Solid-State NMR Compositional Analysis of Suberin-Enriched Wound-Healing Tissues

J Agric Food Chem. 2015 Aug 5;63(30):6810-22. doi: 10.1021/acs.jafc.5b03206. Epub 2015 Jul 24.


The cultivation, storage, and distribution of potato tubers are compromised by mechanical damage and suboptimal healing. To investigate wound-healing progress in cultivars with contrasting russeting patterns, metabolite profiles reported previously for polar tissue extracts were complemented by GC/MS measurements for nonpolar extracts and quantitative (13)C NMR of interfacial solid suspensions. Potential marker compounds that distinguish cultivar type and wound-healing time point included fatty acids, fatty alcohols, alkanes, glyceryl esters, α,ω-fatty diacids, and hydroxyfatty acids. The abundant long-chain fatty acids in nonpolar extracts and solids from the smooth-skinned Yukon Gold cultivar suggested extensive suberin biopolymer formation; this hypothesis was supported by high proportions of arenes, alkenes, and carbonyl groups in the solid and among the polar markers. The absence of many potential marker classes in nonpolar Atlantic extracts and interfacial solids suggested a limited extent of suberization. Modest scavenging activities of all nonpolar extracts indicate that the majority of antioxidants produced in response to wounding are polar.

Keywords: ABTS•+ scavenging activity; GC/MS; Solanum tuberosum; closing layer; potential markers; solid-state NMR; suberin; wound periderm.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antioxidants / analysis*
  • Antioxidants / metabolism
  • Lipids / analysis*
  • Lipids / biosynthesis
  • Magnetic Resonance Spectroscopy
  • Plant Tubers / chemistry*
  • Plant Tubers / physiology
  • Solanum tuberosum / chemistry
  • Solanum tuberosum / classification
  • Solanum tuberosum / physiology*
  • Wound Healing*


  • Antioxidants
  • Lipids
  • suberin