Monitoring disease progression and treatment efficacy with circulating tumor cells in esophageal squamous cell carcinoma: A case report

World J Gastroenterol. 2015 Jul 7;21(25):7921-8. doi: 10.3748/wjg.v21.i25.7921.


This study investigated whether changes in circulating tumor cell (CTC) numbers reflect tumor progression and treatment efficacy in esophageal squamous cell carcinoma (ESCC). A 47-year-old male patient with ESCC is presented in this case study. The patient was evaluated for a series of serum tumor markers and subjected to radiological examinations before and after surgery and during follow-up over the course of five years. In addition, the CTCs in 7.5 mL of peripheral blood were enriched by magnetic-activated cell sorting negative selection and identified by immunofluorescence staining. Serum tumor markers remained within normal ranges and were discordant with imaging scans during the follow-up. Initially, one CTC was detected in the peripheral blood sample, and 14 were observed seven days after the operation. After 12 wk, subcutaneous metastases and bone metastases occurred, and the number of CTCs increased to 84. After 48 wk, lung metastases were noted, and the CTC level was 21. At 104 wk, the number of CTCs was 14, and disease recurrence was detected by positron emission tomography-computed tomography. The CTC counts were in accord with the imaging studies at several time points. The additional information provided by CTC enumeration could thus facilitate monitoring of disease status and treatment efficacy and provide support for treatment decisions.

Keywords: Adjuvant therapy; Circulating tumor cells; Esophageal squamous cell carcinoma; Individualized treatment.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / blood*
  • Biopsy
  • Bone Neoplasms / blood
  • Bone Neoplasms / secondary
  • Carcinoma, Squamous Cell / blood
  • Carcinoma, Squamous Cell / secondary
  • Carcinoma, Squamous Cell / surgery*
  • Chemoradiotherapy, Adjuvant
  • Disease Progression
  • Esophageal Neoplasms / blood
  • Esophageal Neoplasms / pathology
  • Esophageal Neoplasms / surgery*
  • Esophageal Squamous Cell Carcinoma
  • Esophagectomy*
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • Immunomagnetic Separation
  • Lung Neoplasms / blood
  • Lung Neoplasms / secondary
  • Male
  • Middle Aged
  • Neoplastic Cells, Circulating / metabolism*
  • Neoplastic Cells, Circulating / pathology
  • Positron-Emission Tomography
  • Predictive Value of Tests
  • Time Factors
  • Tomography, X-Ray Computed
  • Treatment Outcome


  • Biomarkers, Tumor