Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

J Vis Exp. 2015 Jul 6;(101):e52717. doi: 10.3791/52717.


Halitosis is a common complaint involving social and communicational problems in humans and also affects the pet-owner relationship. In this randomized placebo-controlled crossover clinical evaluation, we assessed the effectiveness of a dedicated dietary supplement to improve chronic halitosis in 32 dogs of different breeds and ages. This protocol describes how to evalute the presence of oral volatile suphur compunds, e.g. methyl mercaptan, hydrogen sulfide and dimethyl sulfide, by means of a portable gas chromatograph device coupled with a syringe, which was used to collect the breath, and a dedicated software, which allows the operator to monitor each compound concentration during each measurement, in a relatively short time (8 min). A significant modification of halitosis parameters was observed after 30 days since the beginning of treatment (p <0.05), while a long-lasting effect was still observed even 20 days after the suspension of the treatment. Portable gas chromatograph, which is also widely used in clinical practice, can be therefore used to confirm and control halitosis in humans and animals. Even though human and animal species present some differences, this innovative and alternative therapy for halitosis management might be extended to human clinical practice as an adjuvant dietary approach.

Publication types

  • Randomized Controlled Trial
  • Video-Audio Media

MeSH terms

  • Animal Feed
  • Animals
  • Breath Tests / methods
  • Chromatography, Gas / methods
  • Cross-Over Studies
  • Dietary Supplements*
  • Dog Diseases / diagnosis*
  • Dog Diseases / drug therapy*
  • Dogs
  • Female
  • Halitosis / diagnosis
  • Halitosis / drug therapy
  • Halitosis / veterinary*
  • Hydrogen Sulfide / analysis
  • Male
  • Sulfhydryl Compounds / analysis
  • Sulfides / analysis


  • Sulfhydryl Compounds
  • Sulfides
  • methylmercaptan
  • dimethyl sulfide
  • Hydrogen Sulfide