Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages

Yu Sumiya; Mami Ishikawa; Takahiro Inoue; Toshio Inui; Daisuke Kuchiike; Kentaro Kubo; Yoshihiro Uto; Takahito Nishikata

Macrophage Activation Mechanisms in Human Monocytic Cell Line-derived Macrophages

Anticancer Res. 2015 Aug;35(8):4447-51.

Authors

Yu Sumiya¹, Mami Ishikawa¹, Takahiro Inoue¹, Toshio Inui², Daisuke Kuchiike³, Kentaro Kubo⁴, Yoshihiro Uto⁵, Takahito Nishikata⁶

Affiliations

¹ Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan.
² Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan Inui Immunotherapy Clinic, Osaka, Japan.
³ Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan Saisei Mirai Cell Processing Center, Osaka, Japan.
⁴ Saisei Mirai Cell Processing Center, Osaka, Japan.
⁵ Department of Life System, Institute of Technology and Science, Graduate School, Tokushima University, Tokushima, Japan.
⁶ Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe, Japan nisikata@konan-u.ac.jp.

PMID: 26168485

Abstract

Background: Although the mechanisms of macrophage activation are important for cancer immunotherapy, they are poorly understood. Recently, easy and robust assay systems for assessing the macrophage-activating factor (MAF) using monocytic cell line-derived macrophages were established.

Materials and methods: Gene-expression profiles of U937- and THP-1-derived macrophages were compared using gene expression microarray analysis and their responses against several MAFs were examined by in vitro experiments.

Results: Activated states of these macrophages could not be assigned to a specific sub-type but showed, however, different unique characteristics.

Conclusion: The unique of monocytic cell line-derived macrophages could provide clues to understand the activation mechanism of macrophages and, therefore, help to develop effective cancer immunotherapy with MAFs.

Keywords: Immunotherapy; THP-1 cell line; U937 cell line; macrophage-activating factor; phagocytic activity.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay / methods
Cell Line
Cytokines / immunology
Gene Expression / genetics
Gene Expression Profiling
Humans
Immunotherapy
Interferon-gamma / immunology
Interleukin-10 / immunology
Interleukin-4 / immunology
Lipopolysaccharides / immunology
Macrophage Activation / genetics
Macrophage Activation / immunology*
Macrophage-Activating Factors / immunology*
Macrophages / cytology
Macrophages / immunology*
Microarray Analysis
Neoplasms / immunology*
Neoplasms / therapy*
Phagocytosis / immunology*
Signal Transduction / immunology
U937 Cells

Substances

Cytokines
IL10 protein, human
IL4 protein, human
Lipopolysaccharides
Macrophage-Activating Factors
Interleukin-10
Interleukin-4
Interferon-gamma