A tomato phloem-mobile protein regulates the shoot-to-root ratio by mediating the auxin response in distant organs

Plant J. 2015 Sep;83(5):853-63. doi: 10.1111/tpj.12932. Epub 2015 Aug 1.

Abstract

The plant vascular system serves as a conduit for delivery of both nutrients and signaling molecules to various distantly located organs. The anucleate sieve tube system of the angiosperm phloem delivers sugars and amino acids to developing organs, and has recently been shown to contain a unique population of RNA and proteins. Grafting studies have established that a number of these macromolecules are capable of moving long distances between tissues, thus providing support for operation of a phloem-mediated inter-organ communication network. Currently, our knowledge of the roles played by such phloem-borne macromolecules is in its infancy. Here, we show that, in tomato, translocation of a phloem-mobile cyclophilin, SlCyp1, from a wild-type scion into a mutant rootstock results in restoration of vascular development and lateral root initiation. This process occurs through reactivation of auxin response pathways and reprogramming of the root transcriptome. Moreover, we show that long-distance trafficking of SlCyp1 is associated with regulation of the shoot-to-root ratio in response to changing light intensities, by modulating root growth. We conclude that long-distance trafficking of SlCyp1 acts as a rheostat to control the shoot-to-root ratio, by mediating root development to integrate photosynthesis and light intensity with requirements for access to water and mineral nutrients.

Keywords: Solanum lycopersicum; auxin; cyclophilin; diageotropica; phloem; root development; shoot-to-root ratio.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biological Transport
  • Cyclophilins / genetics
  • Cyclophilins / metabolism*
  • Gene Expression Regulation, Plant
  • Indoleacetic Acids / metabolism*
  • Lycopersicon esculentum / genetics
  • Lycopersicon esculentum / metabolism*
  • Mutation
  • Phloem / genetics
  • Phloem / metabolism*
  • Photosynthesis / physiology
  • Plant Proteins / metabolism*
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Plant Shoots / genetics
  • Plant Shoots / growth & development
  • Plant Shoots / metabolism
  • Plants, Genetically Modified
  • Signal Transduction

Substances

  • Indoleacetic Acids
  • Plant Proteins
  • Cyclophilins