Arginylation regulates purine nucleotide biosynthesis by enhancing the activity of phosphoribosyl pyrophosphate synthase

Nat Commun. 2015 Jul 15;6:7517. doi: 10.1038/ncomms8517.


Protein arginylation is an emerging post-translational modification that targets a number of metabolic enzymes; however, the mechanisms and downstream effects of this modification are unknown. Here we show that lack of arginylation renders cells vulnerable to purine nucleotide synthesis inhibitors and affects the related glycine and serine biosynthesis pathways. We show that the purine nucleotide biosynthesis enzyme PRPS2 is selectively arginylated, unlike its close homologue PRPS1, and that arginylation of PRPS2 directly facilitates its biological activity. Moreover, selective arginylation of PRPS2 but not PRPS1 is regulated through a coding sequence-dependent mechanism that combines elements of mRNA secondary structure with lysine residues encoded near the N-terminus of PRPS1. This mechanism promotes arginylation-specific degradation of PRPS1 and selective retention of arginylated PRPS2 in vivo. We therefore demonstrate that arginylation affects both the activity and stability of a major metabolic enzyme.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoacyltransferases / genetics*
  • Aminoacyltransferases / metabolism
  • Animals
  • Arginine / metabolism*
  • Blotting, Western
  • Cell Line
  • Glycine / biosynthesis
  • HEK293 Cells
  • Humans
  • Lysine / metabolism
  • Mice
  • Mice, Knockout
  • Molecular Structure
  • Protein Processing, Post-Translational
  • Purine Nucleotides / biosynthesis*
  • RNA, Messenger / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribose-Phosphate Pyrophosphokinase / metabolism*
  • Serine / biosynthesis
  • Ubiquitination


  • Purine Nucleotides
  • RNA, Messenger
  • Serine
  • Arginine
  • Aminoacyltransferases
  • arginyltransferase
  • PRPS1 protein, mouse
  • PRPS2 protein, mouse
  • Ribose-Phosphate Pyrophosphokinase
  • Lysine
  • Glycine